Journal of Animal Behaviour and Biometeorology
Journal of Animal Behaviour and Biometeorology
Research Article Open Access

Effects of decoction products of Lavandula angustifolia, Laurus nobilis, and Artemisia herba-alba on depression and anxiety behaviors in Wistar rats

Kamal Elharas, Mohammed Ouhssine

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Medicinal and aromatic plants have very substantial emotional effects on rats, which is part of the current study. Decoction products of three Moroccan plants (Lavandula angustifolia L., Laurus nobilis L., and Artemisia herba-alba) were used to be tested on Wistar rats in the laboratory. The goal was to check if they had an anti-depressant and/or anti-anxiety action on the animals' tests. Wistar Rats were born and bred in the pet store of the Faculty of Science, Kenitra. The anti-depressant and anti-anxiety effects were assessed according to three animal models: Open Field, Elevated Plus-maze for anxiety, and the Forced Swimming animal model for depression. The results showed that drinking water containing plant extracts has anti-depressant and anti-anxiety effects. Rats have overcome depression by reducing downtime during forced swimming. Concerning anti-anxiety, the open-field test showed an increase in the rat’s locomotor activity, which indicates unhesitating exploratory behavior in the form of confident movement through the number of visits to the central part and the total distance crossed. The anxiety reduction was also corroborated by the elevated Plus-maze test, where the rats displayed no aversion to open spaces on account of the prolonged time they spent in the open arms and platform of the raised cross maze. In light of the findings from the experiments conducted in the study, the decoction products of the three plants appear to be viable candidates for the treatment of depression and anxiety.


anxiety, depression, forced swimming, Moroccan plant extracts, open field, raised cross maze


Albrechet-Souza L, Oliveira AR, De Luca MCZ, Tomazini F, Santos NR, Brandao MLA (2005) Comparative study with two types of elevated plus-maze (transparent vs. opaque walls) on the anxiolytic effects of midazolam, onetrial tolerance and fear-induced analgesia. Prog Neuropsychopharmacol Biol Psychiatr 29:571–9.

Alkozei A, Cooper PJ, Creswell C (2014) Emotional reasoning and anxiety sensitivity: associations with social anxiety disorder in childhood. J Affect Disord 152–154:219–228.

Bellakhdar J (1997) La pharmacopée marocaine traditionnelle, dixième édition.

Bourin M, Benoit P, Brid ND, Martine H (2007) Animal models of anxiety in mice; Fundamental & Clinical Pharmacology 21:567–574.

Borsini F, Meli A (1988) Is the forced swimming test a suitable model for revealing antidepressant activity? Psychopharmacology (Berl.) 94:147–160.

Brown TA, O’Leary TA, Barlow DH (2001) Generalized anxiety disorder. In: Barlow DH (ed) Clinical handbook of psychological disorders: A step-by-step treatment manual. 3rd edn. Guiford, New York, pp. 154–208.

Brummelte S, Lieblich SE, Galea LAM (2012) Gestational and postpartum corticosterone exposure to the dam affects behavioral and endocrine outcome of the offspring in a sexually-dimorphic manner. Neuropharmacology 62:406-418.

Budzynska B, Boguszewska-Czubara A, Kruk-Slomka M, Skalicka-Woziniak K, Michalak A, Musik I, Biala G (2013) Effects of imperatorin on nicotine induced anxiety and memory related responses and oxidative stress in mice Physiol Behav 122:46–55.

Caravan I, Sevastre Berghian A, Moldovan R, Decea N, Orasan R, Filip GA (2016) Modulatory effects of caffeine on oxidative stress and anxiety-like behavior in ovariectomized rats. Can. J. Physiol. Pharmacol 94:1–12.

Carbajal D, Ravelo Y,Molina V, Mas R (2009) Arruzazabala ML D-004 a lipid extract from royal palm fruit, exhibits antidepressant effects in the forced swim test and the tail suspension test in mice; Pharmacol Biochem Behav 92:465–468.

Catherine B, Guy G (2001) Measuring normal and pathological anxiety-like behaviour in mice: a review. Behavioural Brain Research 125:141–149.

Czech Donald A (2002) A simple integrated circuit device for measuring distances traveled and determining speed in open-field environments. Pharmacol Biochem Behav 72:73–75.

Cruz AP, Frei F, Graeff FG (1994) Ethopharmacological analysis of rat behavior on the elevated plusmaze. Pharmacol Biochem Behav 49:171–176.

David DJP, Klemenhagen KC, Holick KA, Saxe MD, Craig DA, Swanson C (2007) Efficacy of the MCHR1 antagonist N-[3-(1-{[4-(3,4-difluorophenoxy)phenyl]methyl}(4-piperidyl))-4-methylphenyl]-2-methylpropanamide (SNAP 94847) in mouse models of anxiety and depression following acute and chronic administration is independent of hippocampal neurogenesis. J Pharmacol Exp Ther 19:237-48.

Dawson GR, Rupniak NM, Iversen SD, Curnow R, Stanhope KJ (1995) Lack of effect of CCKB receptor antagonists in ethological and conditioned animal screens for anxiolytic drugs. Psychopharmacology (Berl) 121:109–117.

Elizalde N, Gil-Bea FJ, Raminez MJ, Aisa B, Lasheras B, Del Rio J (2008) long-lasting behavioral effect of antidepressant treatment. psychopharmacology (Berl) 199:1-14.

Espejo EF (1997) Structure of the mouse behaviour on the elevated plus-maze test of anxiety. Behavioural Brain Research 86:105-112.

Fricchione G (2004) Generalized anxiety disorder. N Engl J Med 351:675–682.

Hacène F, Réda D, Bachir A R, Nabila F, Abdelkrim T, Abdel MB (2010) Le kètoconazole antagonise les effets immuno-gonadotropes au test de la nage force´e chez le rat mâle Wistar. Revue canadienne de physiologie et pharmacologie 88:733-744.

Hall CS (1934) Emotional behaviour in the Rat. I. Defecation and urination as mesures of individual differences in emotionality. J.Comp. Psychol 385-403.

Hall CS (1938) The inheritance of emotionality. Sigma Xi Quarterly 26:17-27.

Herskin MS, Jensen KH (2002) Effects of open field testing and associated handling v. handling alone on the adrenocortical reactivity of piglets around weaning. Anim Sci 74:485–491.

Hmamouchi M (1997) Plantes alimentaires, aromatiques, condimentaires, médicinales et toxiques au Maroc. In Heywood (V.H.) Skoula (M.) (Eds.) Identification of wild food and non-food plants of the Mediterranean region. Chania: CIHEAM-IAMC 89-108.

Jan volák et jiří soldata (1997) plantes médicinales;.dixième édition 319 pp.

Karl T, Pabsat R, Stephan VHS (2003) Behavioral phenotyping of mince in pharmacological and toxicological research. EXP. ToxicolPathol 55:69-83.

Katz RJ, Roth KA, Carroll BJ (1987) Acute and chronic stress effects on open field activity in the rat: implications for a model of depression. Neuroscience and bio behavioral reviews 5:247-51.

Kennett GA, Dourish CT, Curzon G (1987) Antidepressant-like action of 5-HT1A agonists and conventional antidepressants in an animal model of depression. Eur J Pharmacol 134:265–274.

Kevin SL, Craig A, Dana C, Torpey C (2004) Impact of healthy aging on awareness and fear conditioning. Behavioral Neuroscience 118:905-915.

Larrère R (2007) Justifications éthiques des préoccupations concernant le bien-être animal. INRA Prod. Anim 20:11-17.

Luis EG, Sandra EF (1997) A Five Minute Experience in the Elevated Plus-Maze Alters the State of the Benzodiazepine Receptor in the Dorsal Raphe Nucleus, The Journal of Neuroscience 15:1505–1511.

Mansouri MT, Soltani M, Naghizadeh B, Farbood Y, Mashak A, Sarkaki A (2014) A possible mechanism for the anxiolytic-like effect of gallic acid in the rat elevated plus maze. Pharmacol Biochem Behav 117:40–46.

Mohamed B, Hassane M, Abdelkhaleq L, Abderrahim Z (2002) Ethnopharmacology Forum Medicinal plants used in the treatment of diabetes in Morocco; Int J Diabetes & Metabolism 10:33-50.

Mora S, Diaz-Veliz G, Lungenstrass H, Garcia-Gonzalez M, Coto-Morales T, Poletti C (2005) Central nervous system activity of the hydroalcoholic extract of Casimiroa edulis in rats and mice. J Ethnopharmacol 97:191–197.

Pellow S, Chopin P, File SE, Briley M (1985) Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 14:149-167.

Pellow S, Sandra FE (1986) Anxiolytic and anxiogenic drug effects on exploratory activity in an elevated plus-maze: Anovel test of anxiety in the rat. Pharmacol Biochem Behav 24:525–529.

Petit-Demouliere B, Chenu F, Bourin M (2005) Forced swimming test in mice: a review of antidepressant activity. Psychopharmacology 177:245–255.

Porsolt RD, Le Pichon M, Jalfre M (1977) Depression: a new animal model sensitive to antidepressant treatments. Nature 266: 730-732.

Porsolt RD, Anton G, Blavet N et Jalfre M (1978) Behavioral despair in rats: a new model sensitive to antidepressant treatments. Eur. J. Pharmacol 47:379–381.

Porsolt RD, Anton G, Blavet N, Jalfre M (1987) Behavioral despair in rats: a new model sensitive to antidepressant treatments. Eur J Pharmacol. 47:379–391.

Rodgers RJ, Dalvi A (1997) anxiety, defence and the elevated plus-maze. Neuroscience Biobehav 21:801-810.

Saeed SA, Bloch RM, Antonacci DJ (2007) Herbal and Dietary Supplements for Treatment of Anxiety Disorders. Am Fam Physician 76:549–556.

Selye H (1936) A syndrome produced by diverse nocuous agents. Nature 138:32.

Taiwo AE, Leite FB, Lucena GM, Barros M, Silveira D, Silva MV (2012) Anxiolytic and antidepressant-like effects of Melissa officinalis (lemon balm) extract in rats: Influence of administration and gender. Indian J Pharmacol 44:189–192.

Thiebot MH (1989) Les modèles animaux de l’anxiété. Psychologie Médicale 21:97-108.

Van Gaalen MM, Stickler T (2000) Behavioural analysis of four mouse strains in an anxiety test battery. Bahav Brain Res 115:95-109.

Wilner P (1984) The validity of animal models of depression; Psychopharmacology 83:1–16.

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