Journal of Animal Behaviour and Biometeorology
Journal of Animal Behaviour and Biometeorology
Research Article Open Access

Previous sexual experience does not improve estrous behavior and ovulatory activity in seasonally anestrous goats in contact with photo-stimulated bucks

Ilda Graciela Fernández, José Alfredo Flores, Gerardo Duarte, Horacio Hernández, Gonzalo Fitz-Rodríguez, Jesús Vielma

Downloads: 0
Views: 919


Sexually inexperienced ewes display lower estrous behavior during the first contact with rams. The objective of this study was to determine whether sexually inexperienced anestrous females display estrous behavior and ovulatory activity as the sexually experienced females, and with restricted sexual experience exposed to photo-stimulated bucks. Six bucks were rendered sexually active during the non-breeding season by exposure to artificially long days (16 h of light and 8 h of darkness per day) for 2.5 months. The first group, sexually experienced females (n = 10) had physical contact with two vasectomized bucks, and mating was allowed; the second group, with restricted sexual experience (n = 10) had contact with two vasectomized bucks through a wire-mesh fence and mating was prevented; the third group, sexually inexperienced females (n = 9) was totally isolated from bucks. At 14-month-old the females were exposed to photo-stimulated bucks for 15 days. The proportion of females that displayed estrous behavior, ovulations, short and normal ovulatory cycles, and pregnancy rates did not differ between sexually experienced, with restricted sexual experience, and sexually inexperienced (P > 0.05, in all variables). Similarly, the ovulation rate did not differ between the three groups of females (P > 0.05). However, the interval from introduction of the bucks to the first estrus was shorter in sexually inexperienced females (P < 0.05). Therefore, sexually inexperienced anestrous females displayed estrous behavior, ovulation, and pregnancy rate similar to those displayed by sexually experienced and with restricted sexual experience when exposed to photo-stimulated bucks through the male effect.


caprine, male effect, photoperiod, socio-sexual signals


Andrade-Esparza JD, Espinoza-Flores LA, Hernández H, Chemineau P, Keller M, Delgadillo JA (2018) Extensive management conditions do not modify the frequency of short ovulatory cycles in progesterone-treated does exposed to sexually active males. Animal Reproduction Science 199:40-44.

Bedos M, Flores JA, Fitz-Rodríguez G, Keller M, Malpaux B, Poindron P, Delgadillo JA. (2010) Four hours of daily contact with sexually active males is sufficient to induce fertile ovulation in anestrous goats. Hormones and Behavior 58:473-477.

Bedos M, Duarte G, Flores JA, Fitz-Rodríguez G, Hernández H, Vielma J, Fernández IG, Chemineau P, Keller M, Delgadillo JA (2014) Two or 24 h of daily contact with sexually active males results in different profiles of LH secretion that both lead to ovulation in anestrus goats. Domestic Animal Endocrinology 48:93-99.

Chemineau P, Pellicer-Rubio MT, Lassoued N, Khaldi G, Monniaux D (2006) Male-induced short oestrous and ovarian cycles in sheep and goats: a working hypothesis. Reproduction Nutrition and Development 46:417-429.

Delgadillo JA, Canedo GA, Chemineau P, Guillaume D, Malpaux B (1999) Evidence for annual reproductive rhythm independent of food availability in male creole in subtropical northern Mexico. Theriogenology 52:727-737.

 Delgadillo JA, Flores JA, Véliz G, Hernandez H, Duarte G, Vielma J, Poindron P, Chemineau P, Malpaux B (2002) Induction of sexual activity in lactating anovulatory female goats using male goats treated only with artificially long days. Journal of Animal Science 80:2780-2786.

Duarte G, Flores, JA, Malpaux B, Delgadillo JA (2008) Reproductive seasonality in female goats adapted to a subtropical environment persist independently of food availability. Domestic Animal Endocrinology 35:362-370. 

Fatet A, Pellicer-Rubio MT, Leboeuf B (2011). Reproductive cycle of goats. Animal Reproduction Science 124:211-219.   

Fernández IG, Luna-Orozco JR, Vielma J, Duarte G, Hernández H, Flores JA, Gelez H, Delgadillo JA (2011) Lack of sexual experience does not reduce the responses of LH, estrus or fertility in anestrous goats exposed to sexually active males. Hormones and Behavior 60:484-488.

Fernández IG, Flores Medina E, Flores JA, Hernández H, Vielma J, Fitz-Rodríguez G, Duarte G (2018) Absence of previous sexual experience did not modify the response of anoestrous goats to photo-stimulated bucks in Spring. Italian Journal of Animal Science 17:306-311.

Fernández IG, Loya-Carrera J, Sifuentes-Lamónt P, Duarte G, Flores JA, Grimaldo E, Hernández H, Ulloa-Arvizu R, Andrade-Esparza J (2020). Social isolation not inhibit sexual behaviour and testosterone secretion in sexually inexperienced photo-stimulated bucks in contact with seasonally anoestrous goats. Italian Journal of Animal Science 19:989-996.

Gelez H, Lindsay DR, Blanche D, Martin GB, Fabre-Nys C (2003) Temperament and sexual experience affect females sexual behavior in sheep. Applied Animal Behaviour Science 84:81-87.

Gelez H, Archer E, Chesneau D, Lindsay D, Fabre-Nys C (2004) Role of experience in the neuroendocrine control of ewes´ sexual behavior. Hormones and Behavior 45:190-200.

Grajales H, Hernández A, Prieto E. (2010) Niveles de progesterona durante el ciclo normal y silencioso en bovinos en el trópico colombiano. Revista MVZ Córdoba 15:2060-2069.

Hawken PAR, Evans ACO, Beard AP (2008) Prior exposure of maiden ewes to rams enhances their behavioural interactions with rams but is not a pre-requisite to their endocrine response to the ram effect. Animal Reproduction Science 108:13-21.

Hemsworth PH, Cronin GM, Hansen C (1982) The influence of social restriction during rearing on the sexual behavior of the gilt. Animal Production 35:35-40.

Martínez-Alfaro JC, Hernández H, Flores JA, Duarte G, Fitz-Rodríguez G, Fernández IG, Bedos M, Chemineau P, Keller M, Delgadillo JA, Vielma J (2014) Importance of intense male sexual behavior for inducing the preovulatory LH surge and ovulation in seasonally anovulatory female goats. Theriogenology 82:1028-1035.  

Muñoz AL, Bedos M, Aroña RM, Flores JA, Hernández H, Moussu C, Briefer EF, Chemineau P, Keller M, Delgadillo JA (2016) Efficiency of the male effect with photostimulated bucks does not depend on their familiarity with goats. Physiology and Behavior 158:137-142.

Murtagh JJ, Gray SJ, Lindsay DR, Oldham CM (1984) The influence of the ram effect in 10-11 month old Merino ewes on their subsequent performance when introduced to rams again at 15 months of age. Proceedings of the Australian Society of Animal Production 15, 490-493.

Nofrey B, Rocha B, Lopez HH, Ettenber A (2008) The effects of sexual experience and estrus on male-seeking motivated behavior in the female rat. Physiology and Behavior 95:533-538.

Radespiel U, Zimmermann E (2003) The influence of familiarity, age, experience and female mate choice on pregnancies in captive grey mouse lemurs. Behavior. 140:301-318.

Ramírez S, Bedos M, Chasles M, Hernández H, Flores JA, Vielma J, Duarte G, Retana-Márquez MS, Keller M, Chemineau P (2017) Fifteen minutes of daily contact with sexually active male induces ovulation but delays its timing in seasonally anestrous goats. Theriogenology 87:148-153.

Rivas-Muñoz R, Fitz-Rodríguez G, Poindron P, Malpaux B, Delgadillo JA (2007) Stimulation of estrous behavior in grazing female goats by continuous or discontinuous exposure to males. Journal of Animal Science 85:1257-1263.

Rosciszewska ZE (1985) The influence of earlier mating experiencie of ewes on their subsequent mating behaviour and reproductive performance. Animal Reproduction Science 9:223-229.

SAGARPA, Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación. Norma Oficial Mexicana NOM-062-ZOO, Especificaciones técnicas para la producción, cuidado y uso de los animales de laboratorio. Diario Oficial de la Federación, México D.F., 22 Agosto 2001. 

SYSTAT 13.00.05. 2009. Systat Software, Inc., Chicago, IL, USA.

Walkden-Brown SW, Restall BJ, Henniawati (1993) The male effect in the Australian cashmere goat. 2. Role of olfactory cues from the male. Animal Reproduction Science 32:55-67.

Walkden-Brown SW, Restall BJ, Scaramuzzi R J, Martin GB, Blackberry MA (1997) Seasonality in male Australian cashmere goats: long term effects of castration and testosterone or oestradiol treatment on changes in LH, FSH and prolactin concentrations, and body growth. Small Ruminant Research 26:239-252.

Submitted date:

Accepted date:

600c403a0e8825925fe40eaa jabbnet Articles
Links & Downloads

J. Anim. Behav. Biometeorol.

Share this page
Page Sections