Optimization of some environmental and nutritional conditions using microtiter plate for Pseudomonas aeruginosa biofilm formation
Shaymaa Fouad Rasheed Al-Khazraji, Mohammad Abdul Rahmman Al-Maeni
Abstract
One of the most important virulence factors in Pseudomonas aeruginosa is biofilm formation, as it works as a barrier for entering antibiotics into the bacterial cell. Different environmental and nutritional conditions were used to optimize biofilm formation using microtitre plate assay by P. aeruginosa. The low nutrient level of the medium represented by tryptic soy broth (TSB) was better in biofilm formation than the high nutrient level of the medium with Luria Broth (LB). The optimized condition for biofilm production at room temperature (25 °C) is better than at host temperature (37 °C). Moreover, the staining with 0.1% crystal violet and reading the biofilm with wavelength 360 are considered essential factors in increasing the productivity of biofilm formation in P. aeruginosa. Finally, we highly recommended using these optimized microtitre plate assays to assess biofilm formation in P. aeruginosa.
Keywords
References
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Submitted date:
03/20/2021
Reviewed date:
05/30/2021
Accepted date:
06/05/2021
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