Journal of Animal Behaviour and Biometeorology
Journal of Animal Behaviour and Biometeorology
Research Article Open Access

Relationship between glycerol administration to livestock 24 h before sacrifice and indicators of physiological and oxidative stress

Mariana Huerta-Jiménez, María Esther Ortega-Cerrilla, José Guadalupe Herrera-Haro, Jorge R Kawas-Garza, Antonio Díaz-Cruz, Cuauhtémoc Nava, David Hernandez-Sanchez, Eusebio Ortega-Jimenez, Alma Delia Alarcón-Rojo

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The objective of this study was to evaluate the effect of oral administration of glycerol, as a source of energy, to steers 24 h before slaughter on biochemical indicators of physiological and oxidative stress. Fifty Zebu x Swiss- or Simental-cross steers were selected at random at the finalizing stage impending slaughter. Blood samples were collected from the jugular vein before administering the treatments and 24 h afterward. After sacrifice, samples of the Longissimus dorsi muscle were collected. Biochemical indicators evaluated in bovine serum were hematocrit (VGA), lactate dehydrogenase (LDH), lactate (LAC), cortisol (COR), glucose (GLU), β-hydroxybutyrate (BHT), creatine kinase enzyme (CK), free non-esterified fatty acids (NEFA), antioxidant activity (FRAP), thiobarbituric acid reactive substances (TBARS), and glutathione peroxidase reaction (GSH-Px). The variables evaluated in meat were TBARS, FRAP, pH, texture and shelf life. Analysis of the stress biomarkers evaluated in bovine serum did not reveal differences (P>0.05) between treatments T0 and T1 for the variables COR, VGA, GLU, NEFA, BHT or CK. There were, however, differences between sampling times for the variables FRAP (P = 0.021) and GSH (P = 0.006). The indicators FRAP and TBARS in the sampled meat were not different (P>0.05) between treatments. Meat pH changed over time (P=0.0002), but not its texture (P>0.05). It is concluded that the administration of glycerol as an energy supplement 24 h before sacrifice did not modify the balance of physiological constants of the evaluated steers, nor did it produce significant changes in shelf life of meat.


carcass quality, stress biomarkers, ruminants


(LI 2018/50)Association of Official Analytical Chemistry (2000) Official methods of analysis 17ed. AOAC. M.D. USA.

Amtmann VA, Gallo C, Van Schaik G, Tadich N (2006) Relationships between ante-mortem handling, blood based stress indicators and carcass pH in steers. Archivos de Medicina Veterinaria 38:259-264.

Animal Welfare (Care and Procedures) Regulations (2018) Wellington, New Zealand. Government of New Zealand.

Averós X, Martín S, Riu M, Serratosa J, Gosálvez LF (2008) Stress response of extensively young being transported to growing-finishing farms under Spanish summer commercial conditions. Livestock Science 119:174-182.

Benitez HS and Giraldo VLA (2012) Suplementación con glicerol crudo en ganado de leche: Co-producto de biodiesel evaluado in vitro y en la producción y calidad de la leche en el trópico alto de Antioquía, Colombia (Spanish edition). Editorial Academia Española.

Benzie IF and Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Analytical Biochemistry 239:70-76.

Benzie IF and Strain JJ (1999) Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology 299:15-27.

Botsoglov NA, Fletouris DJ, Papageorgiou JE, Vassilopoulos VN, Mantis AJ, Trakatelliss AG (1994) Rapid, sensitive and specific thiobarbituric acid method for measuring lipid peroxidation in animal tissue, food and feedstuff. Journal of Agricultural and Food Chemistry 42:1931-1937.

Chan WKM, Faustman C and Decker E (1997) Lipid oxidation induced by oxymyoglobin with involvement of H2O2. Meat Science 46:181-190.

Chanjula P, Pakdeechanuan P, Wattanasit S (2014) Effects of dietary crude glycerin supplementation on nutrient digestibility, ruminal fermentation, blood metabolites, and nitrogen balance of goats. Asian-Australasian Journal of Animal Sciences 27:365-374.

Coskun B, Inal F, Gurbuz E, Polat ES, Alatas MS (2012) The effects of additional glycerol in different feed form on dairy cows. Kafkas Universitesi Veteriner Fakultesi Dergisi 18:115-120.

Cunningham JG (1999) Textbook of Veterinary Physiology. 2a ed. Ed. Interamericana McGraw-Hill. México.

Damtew A, Yidersal Erega Y, Ebrahim H, Tsegaye S, Msigie D (2018) The effect of long distance transportation stress on cattle: a review. Biomedical Journal Scientific & Technical Research 3:1-5.

DeFrain JM, Hippen AR, Kalscheur KF and Jardon PW (2004) Feeding glycerol to transition dairy cows: Effects on blood metabolites and lactation performance. Journal Dairy Science 87:4195-4206.

Donkin SS (2008) Glycerol from biodiesel production: the new corn for dairy cattle. Revista Brasileira de Zootecnia 37:280-286.

Granja-Salcedo YT, Messana JD, de Souza VC, Dias AVL, Kishi LT, Rebelo LR, Berchielli TT (2017) Effects of partial replacement of maize in the diet with crude glycerin and/or soyabean oil on ruminal fermentation and microbial population in Nellore steers.  British Journal of Nutrition 118:651-660.

Gregory NG (1998) Animal Welfare and Meat Science (Chapter 2 and 4). UK: CABI Publishing.

Gutteridge JM (1975) The use of standards for Malonyldialdehyde. Analytical Biochemistry 69:518-526.

Kang HJ, Piao MY, Kim HJ, Baik M (2017) Effects of ambient temperature and glycerol supplementation on growth performance in Korean cattle steers. Journal of Animal Science 95:298.

Karami‐Shabankareh H, Kafilzadeh F, Piri V, Mohammadi H (2013) Effects of Feeding Dry Glycerol to Primiparous Holstein Dairy Cows on Follicular Development, Reproductive Performance and Metabolic Parameters Related to Fertility during the Early Post‐partum Period. Reproduction in Domestic Animals 48:945-953.

Kim JH, Seo S, Kim CH, Kim JW, Lee BB, Lee Gil, Shin HS, Kim MC, Kil DY (2013) Effect of dietary supplementation of crude glycerol or tallow on intestinal transit time and utilization of energy and nutrients in diets fed to broiler chickens. Livestock Science 154:165-168.

Knowles TG and Warriss PD (2000) Stress physiology of animals during transport In Livestock Handling Transport 2nd edition. Grandin T. (Ed). CABI International. New York.

Lawrence RA, Burk RF (1976) Gluthatione peroxidasa activity in selenium deficient rat liver. Biochemical Biophysical Research Communications 71:952-958.

Macarena E, Linares MB, Hernández F, Madrid J, Garrido MD (2015) Pre-slaughter administration of glycerol as carbohydrate precursor and osmotic agent to improve carcass and beef quality. Livestock Science 182:1-7.

Mach N, Bach A, Devant M (2009) Effects of crude glycerin supplementation on performance and meat quality of Holstein bulls fed high-concentrate diets. Journal of Animal Science 87:632-638.

Madrid J, Villodre C, Valera L, Orengo J, Martínez S, López MJ, Megías MD, Hernández F (2013) Effect of crude glycerin on feed manufacturing, growth performance, plasma metabolites, and nutrient digestibility of growing-finishing pigs. Journal of Animal Science 91:3788-3795.

María GA, Villarroel M, Sañudo C, Olleta JL, Gebresenbet G (2003) Effect of transport time and ageing on aspects of beef quality. Meat Science 65:1335-1340.

Miranda-de la Lama GC, Villarroel M, Olleta JL, Alierta S, Sañudo C and Maria GA (2009) Effect of the pre-slaughter logistic chain on meat quality of lambs. Meat Science 83:604-609.

Monahan FJ (2000) Oxidation of lipids in muscle foods: Fundamental and applied concerns. In E.A. Decker C Faustman and CJ Lopez-Bote (Eds.), Antioxidants in muscle foods: Nutritional strategies to improve quality. New York: John Wiley and Sons, Inc.

Njisane YZ and Muchenje V (2017) Farm to abattoir conditions, animal factors and their subsequent effects on cattle behavioural responses and beef quality — A review. Asian-Australasian Journal Animal Science 30:755-764.

Parker AJ, Dobson GP and Fitzpatrick LA (2007) Physiological and metabolic effects of prophylactic treatment with the osmolytes glycerol and betaine on Bos indicus steers during long duration transportation. Journal Animal Science 85:2916-2923.

Romero PHM, Uribe-Velásquez FL and Sánchez VJ (2011) Stress biomarkers as indicators of animal welfare in cattle beef farming. Biosalud 10:71-87.

Saleem A.M, Zanouny AI, Singar AM (2018) Effect of glycerol supplementation during early lactation on milk yield, milk composition, nutrient digestibility and blood metabolites of dairy buffaloes. Animal 12:757-763.

Saleem, A.M and Singer AM (2018) Growth performance and digestion of growing lambs fed diets supplemented with glycerol. Animal 12:959-963.

SAS, Statistical Analysis System for Windows 9 (2000) Institute Cary, NC, USA.

Šímová V, Večerek V, Passantino A, Voslarova E (2016) Pre-transport factors affecting the welfare of cattle during road transport for slaughter - A review. Acta Veterinaria Brno 85:303-318.

Socreppa LM, Moraes KAK, Oliveira AS, Batista ED, Drosghic LCAB, Botini LA, Paula DC, Stinguel H, Bento FC, Moraes EHBK (2017) Crude glycerine as an alternative energy feedstuff for beef cattle grazing tropical pasture. Journal of Agricultural Science 155:1-8.

Soohyun Cho, Geunho K, Pil-Nam S, Beomyoung P and Sun Moon, K (2015) Effect of slaughter age on the antioxidant enzyme activity, color, and oxidative stability of Korean Hanwoo (Bos taurus coreanae) cow beef. Meat Science 108:44-49.

Volpi-Lagreca G, and Duckett SK (2017) Supplementation of glycerol or fructose via drinking water to grazing lambs on tissue glycogen level and lipogenesis. Journal of Animal Science 95:2558-2575.

Wang C, Liu Q, Huo WJ, Yang WZ, Dong KH, Huang YX and Guo G (2009a) Effects of glycerol on rumen fermentation, urinary excretion of purine derivatives and feed digestibility in steers. Livestock Science 121:15-20.

Wang C, Liu Q, Yang WZ, Yang XM, Huo WJ, Dong KH, Huang YX, He DC (2009b) Effects of glycerol on lactation performance, energy balance and metabolites in early lactation Holstein dairy cows. Animal Feed Science and Technology 151:12-20.

Werner Omazic A, Tråvén M, Bertilsson J, Holtenius K (2013) High- and low-purity glycerine supplementation to dairy cows in early lactation: effects on silage intake, milk production and metabolism. Animal 7:1479-1485.

Xiong YL, Mullins OE, Stika JF, Chen J, Blanchard SP and Moody WG (2007) Tenderness and oxidative stability of post-mortem muscle from mature cows of various ages. Meat Science 77:105-113.

Young J, Karlsson A, Henckel P (2004) Water-holding capacity in chicken breast muscle is enhanced by pyruvate and reduced by creatine supplements. Poultry Science 83: 400-405.

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